Coding
Part:BBa_K738004:Design
Designed by: Huachun Liu Group: iGEM12_ZJU-China (2012-08-09)
FA-2X-MS2: Split GFP N-terminal domain fused with MS2 protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 798
Illegal EcoRI site found at 1182 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 835
Illegal BsaI site found at 1219
Design Notes
MS2 is a dimer.
Source
Dr. Camille J. Delebecque offered us plasmids with MS2 secquence.